The Research Assistant I is part of the Cell and Developmental Biology Department within the School of Medicine Pod 2 at Vanderbilt University and is a key individual contributor responsible for assisting in generating single-cell RNA-sequencing data from a variety of tissues. This would include but not limited to preparing single cell suspensions from tissues, operating the single-cell RNA-seq instrument, and preparing and QC'ing resulting libraries generated. Reporting directly to the lab manager, the Research Assistant interacts with faculty, staff and students within the laboratory.
The lab is broadly interested in understanding how niche signals are integrated and interpreted to result in cell fate decisions, such as cellular decisions to differentiate into one cell type versus another. The lab utilizes single approaches such as DISSECT-CyTOF (Simmons et al., 2015), multiplex imaging (MxIF) (McKinley et al., 2017), and single-cell RNA-seq to query single-cell states. We aim to unravel the degree of heterogeneity and plasticity of epithelial cell populations, such as cancer stem cells, in vivo, and to translate such knowledge into therapeutically tractable targets.
The Department of Cell and Developmental Biology is a vibrant, interdisciplinary environment for cutting-edge research over a scale that spans seven powers of ten, from single molecules to whole organisms. The difference between a test tube and a cell is spatio-temporal organization, and we study molecular, cellular and tissue organization in many of the laboratories within our department, seeking insights into fundamental biological questions and human disease. We are an interactive and highly collaborative department, with a strong graduate student association, outstanding core facilities, a top-ranked developmental biology program, and exceptionally strong faculty. Recent faculty recruits are studying cell migration using super-resolution microscopy, single molecule analysis of microtubule dynamics, systems biology of intestinal epithelia, stem cell biology, and the regulation of plasma membrane composition and ageing.
Duties and responsibilities
Conducting wet lab experiments by preparing single cell suspensions from human biopsies
Setup and operation of single-cell instruments
Assist in adoption and development of various novel approaches for single-cell RNA-seq, including fixation and preservation procedures, cell hashing, antibody barcoding
Day-to-day lab management to ensure efficiency (i.e. maintaining instruments and ordering supplies)
Routine lab work such as buffer stock preparation and aliquoting reagents
Profile ofan Ideal Candidate
A Bachelor's degree in science from an accredited institution of higher education is necessary.
Ability to interact with students, faculty and/or staff in a team environment is necessary.
Outstanding organizational skills, attention to detail, teamwork and communication skills are necessary.
Experience with mammalian cell culture is strongly preferred.
Experience working with tissues is strongly preferred.
Knowledge of laboratory and experimental protocols and procedures is preferred.
Ability to utilize, calibrate, configure and/or troubleshoot laboratory testing systems and instruments is preferred.
Knowledge of sterile procedures is preferred.
About Vanderbilt Benefits
Vanderbilt University offers a competitive, flexiblebenefits package including health, dental, vision, life, accidental death &dismemberment, disability insurance, paid time off, and a 403(b) retirementplan with employer match. Vanderbilt offers tuition assistance to employees,spouses, and dependent children.
VanderbiltUniversity, located in Nashville, Tennessee, is a top-15 private researchuniversity offering a full range of undergraduate, graduate and professionaldegrees. Vanderbilt is situated on a 330-acre campus near the thriving citycenter, serving more than 12,000 students and employing almost 7,000 facultyand staff. The university's students, staff, and faculty frequently citeNashville and the surrounding area as one of the many perks of being a part ofthe Vanderbilt community. Vanderbilt University is a place where your expertisewill be valued, your knowledge expanded, and your abilities challenged. It is aplace where your diversity is sought and celebrated. Vanderbilt was recentlynamed as one of "America's Best Large Employers" and the top employer inTennessee and the Nashville metropolitan area in 2019 (Forbes).
Commitmentto Equity, Diversity and Inclusion
Vanderbilt University is committed to achieving thegoal of a diverse and inclusive academic community of faculty, staff, andstudents. We seek individuals who are committed to this goal and our campusvalues.
Internal Number: 1900888
About Vanderbilt University
Vanderbilt University is a center for scholarly research, informed and creative teaching, and service to the community and society at large. Vanderbilt will uphold the highest standards and be a leader in the quest for new knowledge through scholarship, the dissemination of knowledge through teaching and outreach, and the creative experimentation of ideas and concepts. In pursuit of these goals, Vanderbilt values most highly intellectual freedom that supports open inquiry, equality, compassion, and excellence in all endeavors.